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KMID : 0603820060120040405
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Journal of Experimental & Biomedical Science
2006 Volume.12 No. 4 p.405 ~ p.411
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The Alpha Subunit of Go Interacts with Brain Specific High Mobility Group Box Containing Protein
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Park Jung-Sik
Ghil Sung-Ho
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Abstract
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Heterotrimeric GTP binding proteins (G proteins) mediate signal transduction generated by neurotransmitter and hormones. Among G-proteins, Go is classified as a member of the Go/Gi family and the most abundant heterotrimeric G protein in brain. Most of the mechanistic analyses on the activation of Go indicated its action to be mediated by the G{beta}{gamma} dimer because downstream effectors for its {alpha} subunit have not been clearly defined. To determine the downstream effectors of alpha subunits of Go (Go{alpha}), we used yeast two-hybrid system to screen Go{alpha} interacting partners in cDNA library from the human brain. A brain specific high mobility group box containing protein (BHX), A possible transcription factor, was identified as a Go{alpha} interacting protein. We confirmed interaction between Go{alpha} and BHX employing in vitro affinity binding assay. Moreover, active form of Go{alpha} preferentially interacts with BHX than inactive farm. Our findings indicate that Go{alpha} could modulate gene expression via interaction with BHX during neuronal or brain development.
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KEYWORD
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G-proteins, High mobility group (HMG), Yeast two hybrid, Affinity binding
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